Analysis of LIMS2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1700-LGZ)

Introduction

Official Full Name: LIM zinc finger domain containing 2<br />Also known as: LGMD2W; PINCH2; MDRCMTT; PINCH-2<br />This gene encodes a member of a small family of focal adhesion proteins that interact with ILK (integrin-linked kinase), a protein that affects the interaction of proteins with the extrabone matrix. The encoded protein has five LIM domains, each of which forms two zinc fingers that allow interactions to regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants of this gene have been found encoding different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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