Analysis of LMX1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0159-LGZ)

Introduction

Also known as: LMX1; DFNA7; LMX1.1<br />This gene encodes a homeodomain and LIM-domain containing protein. This encoded protein is a transcription factor that acts as a positive regulator of insulin gene transcription. The gene also plays a role in the development of dopamine-producing neurons during embryogenesis. Mutations in this gene are associated with an increased risk of Parkinson's disease. Alternate splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Autosomal-Recessive Hereditary Hearing Impairment, Sensorineural hearing loss disorder

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of SCN1A-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1487-LGZ)
Digital Droplet PCR (ddPCR） (CAT#: STEM-MB-0188-WXH)
Analysis of DISC2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0919-LGZ)
Analysis of OR52K1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2367-LGZ)
Analysis of MIR4269 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1636-LGZ)
Analysis of Caprine Arthritis-encephalitis Virus(CAEV) by Real-Time PCR Method (CAT#: STEM-MB-2683-LGZ)
Rapid PIK3CA Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer by RT-qPCR (CAT#: STEM-MT-0018-LGZ)
Analysis of Bunostomum Trigonocephalum by Real-Time PCR Method (CAT#: STEM-MB-2725-LGZ)