Analysis of LOC122889013 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1630-LGZ)

Introduction

Gene description: Sharpr-MPRA regulatory region 7631
Gene type: biological region
This genomic sequence was predicted to be a transcriptional regulatory region based on chromatin state analysis from the ENCODE (ENCyclopedia Of DNA Elements) project. It was validated as a functional repressive element by the Sharpr-MPRA technique (Systematic high-resolution activation and repression profiling with reporter tiling using massively parallel reporter assays) in K562 erythroleukemia cells (group: K562 Repressive non-DNase unmatched - State 23:Low, low signal proximal to active elements).

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LRIG2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1160-LGZ)
Analysis of European Brown Hare Syndrome Virus (EBHSV) by Real-Time PCR Method (CAT#: STEM-MB-3865-LGZ)
Analysis of FUT7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2016-LGZ)
Analysis of Saprolegnia Parasitica by Real-Time PCR Method (CAT#: STEM-MB-3559-LGZ)
Analysis of Strongyloides Ransomi by Real-Time PCR Method (CAT#: STEM-MB-3023-LGZ)
Analysis of Human Papillomavirus 58 (HPV-58) by Real-Time PCR Method (CAT#: STEM-MB-3342-LGZ)
Analysis of Cyclospora Cayetanensis by Real-Time PCR Method (CAT#: STEM-MB-2598-LGZ)
Analysis of Classical Swine Fever Virus-Vaccine(CSFV-Vaccine) by Real-Time PCR Method (CAT#: STEM-MB-2650-LGZ)