Analysis of LOC126860552 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1925-LGZ)

Introduction

Gene description: BRD4-independent group 4 enhancer GRCh37_chr9:285795-286994
Gene type: biological region
This genomic region was validated as an active enhancer by the STARR-seq (self-transcribing active regulatory region sequencing) massively parallel reporter assay in HCT116 colorectal carcinoma cells. This sequence was defined as a group 4 enhancer that depends on the BRD2, P300/CBP, MED14 and CDK7 cofactors, but it has limited or no dependence on the BRD4 bromodomain protein.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LOC126806301 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1457-LGZ)
Analysis of PRRSV-A by Real-Time PCR Method (CAT#: STEM-MB-2920-LGZ)
Analysis of Yellow Fever Virus (YFV) by Real-Time PCR Method (CAT#: STEM-MB-3039-LGZ)
Analysis of Clostridium Sordellii by Real-Time PCR Method (CAT#: STEM-MB-2635-LGZ)
Analysis of CRIM1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1815-LGZ)
Analysis of GON4L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1010-LGZ)
Analysis of SNORD14A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2432-LGZ)
Analysis of Langat Virus by Real-Time PCR Method (CAT#: STEM-MB-3603-LGZ)