Analysis of M-CSF (Mouse) by ELISA (CAT#: STEM-MB-1045-LGZ)

Introduction

M-CSF (macrophage colony-stimulating factor, CSF-1) is an essential survival factor for the proliferation and development of monocyte, macrophage and osteoclast progenitor cells. M-CSF also induces macrophages to secrete VEGF (vascular endothelial growth factor), thereby mediating the mobilization and neovascularization of endothelial progenitor cells. M-CSF exists in several biologically active isoforms of varying potency and stability. The full-length protein is synthesized as a transmembrane protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: plasma, serum, supernatant

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