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Analysis of TNFRSF8/CD30 by ELISA (CAT#: STEM-MB-1543-LGZ)

Introduction

Leukocyte antigen differentiation Cluster 30(CD30), also known as tumor necrosis factor receptor superfamily Member 8 (TNFRSF8), is a membrane protein belonging to the tumor necrosis factor receptor (TNFR) superfamily. CD30 is expressed by activated T and B cells rather than resting cells. CD30 may play a role in the regulation of cell growth and activated lymphoblastic transformation, and may also play an important role in human immunodeficiency virus replication. TRAF2 and TRAF5 can interact with this receptor and mediate signal transduction leading to the activation of NF-κB. As a positive regulator of apoptosis, CD30 can induce cell death or proliferation depending on cell type and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect against autoimmunity. CD30 protein expression is upregulated in various hematological malignancies, and CD30 is also associated with white blood cells in patients with chronic inflammatory diseases such as lupus erythematosus, asthma, and rheumatoid arthritis.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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