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Analysis of MCAM/CD146 (Human) by ELISA (CAT#: STEM-MB-1029-LGZ)

Introduction

CD146, also known as melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a single chain membrane penetrating glycoprotein belonging to the immunoglobulin superfamily (Igsf) and homologous to many cell adhesion molecule (CAM). CD146 is active in a variety of cellular physiological processes, including development, signal transduction, cell migration, mesenchymal stem cell differentiation, angiogenesis, and immune response. Among them, the most studied one is involved in tumor angiogenesis and metastasis.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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