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Analysis of MDC (Non-human primate) by ELISA (CAT#: STEM-MB-1047-LGZ)

Introduction

A cDNA encoding a novel human chemokine was isolated by random sequencing of human monocyte-derived macrophage cDNA clones. This protein is called macrophage-derived chemokine (MDC) because it appears to be synthesized exclusively by cells of the macrophage lineage. MDC has a 4-cysteine motif and other highly conserved residues characteristic of CC chemokines, yet it shares 35% homology with any known chemokine. MDCs are a unique member of the CC chemokine family and may play fundamental roles in the function of dendritic cells, natural killer cells and monocytes.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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