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Analysis of MDM4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0395-LGZ)

Introduction

Official Full Name: MDM4 regulator of p53<br />Also known as: HDMX; MDMX; MRP1; BMFS6<br />The nuclear protein encoded by this gene contains a p53-binding domain at the n-terminus and a RING finger domain at the c-terminus, and its structure is similar to the p53-binding protein MDM2. These two proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 that degrades p53, this protein represses p53 by binding its transcriptional activation domain. The protein also interacts with the MDM2 protein through the RING finger domain, inhibiting the latter's degradation. Thus, this protein can reverse mdm2-targeted p53 degradation while maintaining inhibition of p53 deactivation and apoptotic functions. For this gene, alternatively spliced transcript variants encoding different isoforms have been found.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements