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Melamine is an important nitrogen heterocyclic organic chemical raw material. It is white crystalline powder. Chronic or repeated intake of melamine in large quantities may affect kidneys and bladder, which may lead to stone production. At present, melamine detection methods include: gas chromatography mass spectrometry (GC-MS), liquid chromatography tandem mass spectrometry (LC-MS-MS), liquid chromatography and so on. But the operation is complicated and the cost is high. Compared with instrumental analysis, ELISA has the characteristics of rapid, simple, accurate and sensitive. Can minimize the operation error and work intensity.