Analysis of HB-EGF (Human) by ELISA (CAT#: STEM-MB-0878-LGZ)

Introduction

Heparin-binding epidermal growth factor (HB-EGF) is a member of the EGF family that binds to heparin. It is a potent mitogen and chemokine for fibroblasts and smooth muscle cells. HB-EGF is synthesized in a membrane-anchored form (pro-HB-EGF), which is processed by the ADAM family of metalloproteases to release the soluble mature peptide, a process termed "ectodomain shedding". It is biologically active both as a membrane-anchored form and as a soluble form (s-HB-EGF). HB-EGF is involved in physiological and pathological processes such as wound healing, cardiac hypertrophy, smooth muscle cell proliferation, renal duct morphogenesis, blastocyst implantation, pulmonary hypertension, and tumorigenic transformation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Plasma

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