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Analysis of MERS-CoV Spike (Human) by ELISA (CAT#: STEM-MB-1048-LGZ)

Introduction

Coronaviruses (CoV) are membranous single-stranded plus strand RNA viruses, crustaceans, coronaviruses, family coronaviruses and genus Coronaviruses, which are the largest known RNA viruses. So far, there are seven coronavirus that can infect human beings, namely HCoV-229E and HCoV-NL63 in α group, HCoV-OC43 and HCoV-HKUl in β group A, Atypical pneumonia virus (SARS-CoV) and 2019 Novel coronavirus (2019-NCoV) in B group, Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Group C.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

COVID-19 Coronavirus

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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