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Analysis of MET Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1849-LGZ)

Introduction

Official Full Name: MET proto-oncogene, receptor tyrosine kinase<br />Also known as: DA11; HGFR; AUTS9; RCCP2; c-Met; DFNB97<br />This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. Proteolytic processing of the encoded preproprotein yields the α and β subunits, which are linked by disulfide bonds to form the mature receptor. Further processing of the β subunit leads to the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding to its ligand hepatocyte growth factor induces receptor dimerization and activation, playing a role in cell survival, embryogenesis, cell migration and invasion. Mutations in this gene have been associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene has also been associated with various human cancers.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements