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Analysis of Minimal Residual Disease (MRD) by RT-qPCR (CAT#: STEM-MT-0061-LGZ)

Introduction

In patients with acute myeloid leukemia (AML), treatment stratification is largely based on pretreatment factors, including cytogenetic and molecular aberrations and measurable/minimal residual disease (MRD) during treatment. Sequential MRD monitoring allows assessment of response to chemotherapy and early detection of relapse, potentially identifying patients in need of preemptive or more intensive therapy.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Acute Myeloid Leukemia (AML)

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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