Analysis of MNDA Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0459-LGZ)

Introduction

Official Full Name: myeloid cell nuclear differentiation antigen<br />Also known as: PYHIN3<br />Myeloid nuclear differentiation antigen (MNDA) is only detected in the nucleus of cells of the granulocyte-monocyte lineage. The 200-amino acid region of human MNDA is strikingly similar to protein regions encoded by a family of interferon-induced mouse genes named Ifi-201, Ifi-202, and Ifi-203 that are not regulated in a cell- or tissue-specific manner . The 1.8 kb MNDA mRNA, which contains an interferon-stimulated response element in the 5' untranslated region, is significantly upregulated in human monocytes exposed to interferon-α MNDA is located within 2,200 kb of FCER1A, APCS, CRP and SPTA1. MNDA is similar to IFI16 in its expression and/or regulation pattern, suggesting that these genes are involved in blood cell-specific responses to interferons.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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