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Analysis of MSH6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1141-LGZ)

Introduction

Official Full Name: mutS homolog 6<br />Also known as: GTBP; HSAP; p160; GTMBP; MSH-6; HNPCC5; LYNCH5; MMRCS3<br />This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps recognize mismatched nucleotides before they are repaired. A highly conserved region of approximately 150 aa exists among MutS homologues, called the Walker-A adenine nucleotide-binding motif. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that acts as a bidirectional molecular switch that exchanges ADP and ATP upon DNA mismatch association and dissociation. Mutations in this gene may be associated with hereditary nonpolyposis colon, colorectal, and endometrial cancers. Transcript variants encoding different isoforms have been described.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary nonpolyposis colorectal neoplasms

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements