Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of MSP/MST1/HGFL by ELISA (CAT#: STEM-MB-1547-LGZ)

Introduction

Macrophage stimulating proteins (MSP), also known as hepatocyte growth factor-like proteins (HGFL) and MST1, are members of the HGF family of growth factors. MSP is secreted as inactive single-stranded precursors (pro-MSP) and is a ligand for the transmembrane receptor tyrosine kinase receptor (RON, also known as MST1R). Since its discovery, MSP has been shown to play a key role in regulating inflammation in peripheral tissues in a variety of disease models. Recent evidence also suggests that MSP has a beneficial role in regulating lipid and glucose metabolism in the liver, thereby hinting at MSP as a key regulator in maintaining metabolic homeostasis while inhibiting inflammatory processes. MSP induces macrophage and keratinocyte proliferation and osteoclast activation. It also inhibits LPS or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells isolated from extracellular matrix.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
Advertisement