Analysis of Mycobacterium Asiaticum by Real-Time PCR Method (CAT#: STEM-MB-2947-LGZ)

Introduction

Mycobacterium asiaticum (M. asiaticum) is a species of nontuberculous mycobacteria (NTM) which was first described by Weiszfeiler et al., in 1971. It is a slow growing mycobacterium, closely related to M. simiae.<br />A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.