Analysis of PAPPA2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0534-LGZ)

Introduction

Official Full Name: pappalysin 2<br />Also known as: SSDA; PAPPE; PLAC3; PAPP-E; PAPP-A2<br />This gene encodes a member of the pappalysin family of zincin metalloproteases. This encodes the protein cleavage insulin-like growth factor-binding protein 5, which is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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