Analysis of Mycobacterium Fortuitum by Real-Time PCR Method (CAT#: STEM-MB-2954-LGZ)

Introduction

Mycobacterium fortuitum is an environmental, nonchromogenic, and rapidly growing mycobacteria. Traditionally the M. fortuitum group has included the species: M. fortuitum, Mycobacterium peregrinum and the so-called M. fortuitum group third biovariant complex.<br />A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.