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Analysis of NDUFS2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0155-LGZ)

Introduction

Also known as: CI-49; MC1DN6<br />The protein encoded by this gene is the core subunit of mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I consists of at least 43 distinct subunits, seven of which are encoded by the mitochondrial genome and the rest are products of nuclear genes. The iron-sulfur protein portion of complex I consists of seven subunits, including the gene product. Complex I catalyzes NADH oxidation with concomitant reduction of ubiquinone and extramitochondrial proton ejection. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternative splice transcript variants of this gene have been found to encode distinct isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Leber optic atrophy

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements