Analysis of NEK2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0493-LGZ)

Introduction

Official Full Name: NIMA related kinase 2<br />Also known as: NLK1; RP67; NEK2A; HsPK21; PPP1R111<br />This gene encodes a serine/threonine protein kinase involved in the regulation of mitosis. The protein localizes to the centrosome and is undetectable in G1 phase, but gradually accumulates throughout S phase, reaching maximum levels in late G2 phase. Alternative splice transcript variants of this gene encode different isoforms with different C-termini.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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