Analysis of Neurotrophin-3 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0461-LGZ)

Introduction

Neurotrophin-3, NT-3, is a member of the nerve growth factor family. The sequences of human and rat, mouse and pig are identical. NT-3 plays an important role in the development and maintenance of neural cell populations. Can promote the differentiation of sensory neurons and sympathetic neurons. NT-3 is a dimer composed of 119 amino acid chains with a molecular weight of 27KDa. NT-3 binds p75-NGFR, and initiates apoptosis. Its activating signaling occurs through the TrkC receptor.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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