Analysis of NFKB2 Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0066-LGZ)

Introduction

Official Full Name: nuclear factor kappa B subunit 2
Also known as: p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
This gene encodes a subunit of the transcription factor complex nuclear factor kappa b (NFkB). The NFkB complex is expressed in many cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can act as a transcriptional activator or repressor, depending on its dimerization partner. The full-length p100 protein is co-translationally processed into the active p52 form. Chromosomal rearrangements and translocations at this locus have been observed in B-cell lymphomas, some of which may lead to the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Under certain conditions, two single strands of nucleic acid with certain homology can be specifically hybridized to form double strands according to the principle of base complementarity. Generally, DNA molecules to be detected are digested with restriction enzymes, separated by agar-gel electrophoresis, denatured and transferred to nitrocellulocellulose film or nylon film or other solid phase support according to their position in the gel, fixed and then reacted with DNA probes labeled with isotopes or other markers. This is followed by autoradiography or an enzyme reaction to detect the amount of specific DNA molecules. If the object to be tested contains a sequence that is complementary to the probe, the two are combined by the principle of base complementarity, and the free probe is washed and detected by self-development or other suitable techniques, thus revealing the fragment to be tested and its relative size.

Applications

Gene Rearrangement Detection

Procedure

1. Sample Processing
2. DNA Extraction and Digestion
3. Gel Electrophoresis
4. Gel Pretreatment
5. Transfer membrane
6. Probe Labeling
7. Prehybridization (blocking)
8. Southern hybridization
9. Membrane washing
10. Autoradiographic Assay
11. Results Analysis

Materials

Sample: DNA, Bacterial Fluid/Tissue/Cell

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