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Analysis of β-NGF (Human) by ELISA (CAT#: STEM-MB-1274-LGZ)

Introduction

Nerve growth factor (NGF) is a neuropeptide that is mainly involved in regulating the growth, proliferation and survival of certain target neurons. When expressed, NGF initially exists as a 7S, 130 kDa complex consisting of three proteins, α-NGF, β-NGF, and γ-NGF (2:1:2 ratio). "Nerve growth factor" generally refers to a 2.5S, β subunit protein with a molecular weight of 26 kDa. The β subunit is the only biologically active component (eg, as a signaling molecule) of the 7SNGF complex. NGF plays an important role in both innate and acquired immunity. Studies have shown that NGF circulates in the body through plasma and is important for the maintenance of overall homeostasis. NGF can promote the repair of myelin, and can also participate in a variety of mental diseases, such as Alzheimer's disease, depression, schizophrenia, autism, Rett syndrome, anorexia nervosa, and bulimia nervosa. Dysregulation of NGF signaling is associated with Alzheimer's disease. Likewise, NGF plays a role in many cardiovascular diseases such as coronary atherosclerosis, obesity, type 2 diabetes, metabolic syndrome. NGF also promotes wound healing.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration, Neuroinflammation, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or supernatant