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Analysis of NOS1AP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0481-LGZ)

Introduction

Official Full Name: nitric oxide synthase 1 adaptor protein<br />Also known as: CAPON; NPHS22; 6330408P19Rik<br />The gene encodes a cytosolic protein that binds to the signaling molecule neuronal nitric oxide synthase (nNOS). The protein has a C-terminal PDZ-binding domain that mediates interactions with nNOS, and an N-terminal phosphotyrosine-binding domain (PTB) that binds to the small monomeric G protein Dexras1. Studies of the associated protein in mice and rats revealed that the protein acts as an adapter protein that connects nNOS to specific targets such as Dexras1 and synapses. Alternative splicing results in multiple transcript variants encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements