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Analysis of NPPB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1348-LGZ)

Introduction

Official Full Name: natriuretic peptide B <br />Also known as: BNP; Iso-ANP<br />This gene is a member of the natriuretic peptide family and encodes a secreted protein with cardiac hormone function. This protein undergoes two divisions, one inside the cell and another after it is secreted into the blood. Biological actions of this protein include natriuresis, diuresis, vasodilation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. High levels of this protein in the blood indicate heart failure. The presence of myocardial injury is an important predictor of mortality in hospitalized patients with coronavirus disease 2019 (COVID-19), and there is evidence of elevated levels of natriuretic peptide B in hospitalized nonsurvivor COVID-19 patients. The protein also has antibacterial and antifungal activity as an antimicrobial peptide. Mutations in this gene have been linked to postmenopausal osteoporosis.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements