Analysis of NTRK1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0150-LGZ)

Introduction

Also known as: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA<br />This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon binding of neurotrophins, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been linked to congenital pain insensitivity, anhidrosis, self-injurious behavior, cognitive impairment and cancer. Other transcriptional splice variants of this gene have been identified, but only three have been characterized to date.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary insensitivity to pain with anhidrosis

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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