Detection of p53 Gene Mutations in Small Cell Lung Cancer by RT-qPCR (CAT#: STEM-MT-0032-LGZ)

Introduction

Recent studies suggest that the p53 cancer protein may function properly as a tumor suppressor. Mutations in highly conserved regions of the p53 gene have been observed in many types of tumors and tumor cell lines. We can test for p53 mutations in small cell lung cancer (SCLC).

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Small Cell Lung Cancer

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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