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Analysis of RFX5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0598-LGZ)

Introduction

Official Full Name: regulatory factor X5<br />Lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or bare lymphocyte syndrome (BLS). At least four complementation groups were identified in B-cell lines established from BLS patients. Molecular defects in complementary groups B, C, and D all result in loss of RFX, a nucleoprotein complex that binds the MHC-II promoter X box. The lack of RFX-binding activity of complementation group C is due to mutations in the RFX5 gene encoding the 75-kD subunit of RFX. RFX5 is the fifth member of a growing family of DNA-binding proteins that share a novel and highly characterized DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been identified, but the full-length nature of only two has been determined.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements