Analysis of OR52D1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2342-LGZ)

Introduction

Official Full Name: olfactory receptor family 52 subfamily D member 1<br />Also known as: OR11-43<br />Olfactory receptors interact with odor molecules in the nose, initiating neuronal responses that trigger the perception of odors. Olfactory receptor proteins are members of a large family of G protein-coupled receptors (GPCRs) generated by single-coding exon genes. Olfactory receptors share a 7-transmembrane domain with many neurotransmitter and hormone receptors and are responsible for odorant signal recognition and G protein-mediated transduction. The Olfactory receptor gene family is the largest in the genome. The nomenclature of the olfactory receptor genes and proteins of this organism is independent of that of other organisms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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