Analysis of Orientia Tsutsugamushi by Real-Time PCR Method (CAT#: STEM-MB-3515-LGZ)

Introduction

Orientia tsutsugamushi is an obligate intracellular bacteria and the causative agent of scrub typhus endemic in the Asian-Pacific region. This disease is one of the main causes of acute febrile illness in endemic regions and has drawn much attention from public health agencies due to its recent emergence and continuous local outbreaks.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.