Analysis of p38 (Human, Mouse, Rat) by ELISA (CAT#: STEM-MB-1113-LGZ)

Introduction

p38 mitogen-activated protein kinase (p38MAPK), which first came to attention as a stress-related kinase, has recently been found to be involved in a variety of tumors, In cervical cancer, ovarian cancer, liver cancer and lymphoma, it is closely related to the initiation of apoptosis and the quiescence of cell cycle, and has cell specificity. In different tumor cells, it has different effects, or even completely opposite effects. Therefore, specific research on the role of p38MAPK signaling pathway in various tumors and normal cells can provide theoretical guidance for further tumor therapy.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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