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Analysis of PARP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0564-LGZ)

Introduction

Official Full Name: poly(ADP-ribose) polymerase 1
Also known as: PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP
This gene encodes the chromatin-associated enzyme poly(ADP-ribosyl)transferase, which modifies various nucleoproteins with poly(ADP-ribosyl). This modification is DNA-dependent and involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation, as well as molecular events that regulate cellular recovery from DNA damage. In addition, this enzyme may be the mutation site of Fanconi anemia and may be involved in the pathophysiology of type 1 diabetes.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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