Analysis of CD53 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1174-LGZ)

Introduction

Official Full Name: CD53 molecule
Also known as: MOX44; TSPAN25
The protein encoded by this gene is a member of the transmembrane superfamily, also known as the tetraprotein family. Most of these members are cell surface proteins characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events and play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein known to complex with integrins. It contributes to the transduction of CD2-producing signals in T cells and natural killer cells and is thought to play a role in growth regulation. Familial deletion of this gene is associated with immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi, and viruses. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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