Analysis of Pasteurella Spp. by Real-Time PCR Method (CAT#: STEM-MB-3626-LGZ)

Introduction

Pasteurella spp. are small, pleomorphic, nonmotile, non-spore-forming, Gram-negative coccobacilli. P. multocida is facultatively anaerobic, grows well on blood agar, chocolate agar, Mueller-Hinton agar and brain-heart infusion (BHI), but it shows no growth on MacConkey agar.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.