Analysis of PDC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0558-LGZ)

Introduction

Official Full Name: phosducin
Also known as: PHD; MEKA; PhLP; PhLOP
This gene encodes a phosphoprotein that is located in the inner and outer segments of retinal rod cells. This protein may be involved in the regulation of visual light transduction or in the integration of photoreceptor metabolism. It regulates the phototransduction cascade by interacting with the β and γ subunits of the retinal G-protein transducin. This gene is a potential candidate gene for retinitis pigmentosa and Usher syndrome type II. Alternative splicing transcript variants encoding different isoforms have been identified.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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