Analysis of Pentraxin 3 (Human) by ELISA (CAT#: STEM-MB-1125-LGZ)

Introduction

Pentraxin 3 (PTX-3) belongs to the pentraxin superfamily, which is an important part of the human innate immune system and plays an important role in human vascular biology. Like short pentraxin, PTX3 plays a role in promoting the dysregulation of mitotic signaling pathways, maintaining cell proliferation, angiogenesis, insensitivity to apoptosis, invasion and migration of tumor cells, and immune evasion of tumors. Unlike short pentraxin, PTX3 is produced by a variety of cells at sites of inflammation. Prostate biopsy results confirmed that 80% of patients had chronic inflammation, and PTX3 seemed to be associated with inflammation-related carcinogenesis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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