Analysis of Pik3r1 Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0041-LGZ)

Introduction

Official Full Name: phosphoinositide-3-kinase regulatory subunit 1
Also known as: PI3K; p50alpha; p55alpha; p85alpha
Insulin receptor substrate binding activity; phosphatidylinositol 3-kinase regulatory subunit binding activity; and protein heterodimerization activity. Involved in multiple processes, including negative regulation of stress fiber assembly; positive regulation of cellular component organization; positive regulation of protein import into the nucleus. Acts upstream or within several processes, including apoptotic signaling; negative regulation of osteoclast differentiation; positive regulation of tumor necrosis factor production. Located in several cellular components, including cell-cell junctions; the cis-Golgi network; and the perinuclear endoplasmic reticulum membrane. Some contain protein complexes. Expressed in several structures, including the digestive system; brain; eyes; genitourinary system; and integrative system. For the study of X-linked aglobulinemia. The human homologue of this gene has been associated with several diseases, including SHORT syndrome; astroblastoma; endometrial cancer (multiple); immunodeficiency; and type 2 diabetes. Homologous to human PIK3R1 (phosphoinosine-3-kinase regulatory subunit 1).

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Principle Applications Procedure Materials Notes Related Products

Principle

Under certain conditions, two single strands of nucleic acid with certain homology can be specifically hybridized to form double strands according to the principle of base complementarity. Generally, DNA molecules to be detected are digested with restriction enzymes, separated by agar-gel electrophoresis, denatured and transferred to nitrocellulocellulose film or nylon film or other solid phase support according to their position in the gel, fixed and then reacted with DNA probes labeled with isotopes or other markers. This is followed by autoradiography or an enzyme reaction to detect the amount of specific DNA molecules. If the object to be tested contains a sequence that is complementary to the probe, the two are combined by the principle of base complementarity, and the free probe is washed and detected by self-development or other suitable techniques, thus revealing the fragment to be tested and its relative size.

Applications

Gene Rearrangement Detection

Procedure

1. Sample Processing
2. DNA Extraction and Digestion
3. Gel Electrophoresis
4. Gel Pretreatment
5. Transfer membrane
6. Probe Labeling
7. Prehybridization (blocking)
8. Southern hybridization
9. Membrane washing
10. Autoradiographic Assay
11. Results Analysis

Materials

Sample: DNA, Bacterial Fluid/Tissue/Cell

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