Analysis of MAML2 Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0020-LGZ)

Introduction

Official Full Name: mastermind like transcriptional coactivator 2
Also known as: MAM2; MAM3; MAM-3; MLL-MAML2
The protein encoded by this gene is a member of the Mastermind-like family of proteins. All family members are proline and glutamine-rich, and contain a conserved basic domain that binds the ankyrin repeat domain of the intracellular domain of the Notch receptors (ICN1-4) in their N-terminus, and a transcriptional activation domain in their C-terminus. This protein binds to an extended groove that is formed by the interaction of CBF1, Suppressor of Hairless, LAG-1 (CSL) with ICN, and positively regulates Notch signaling. High levels of expression of this gene have been observed in several B cell-derived lymphomas. Translocations resulting in fusion proteins with both CRTC1 and CRTC3 have been implicated in the development of mucoepidermoid carcinomas, while a translocation event with CXCR4 has been linked with chronic lymphocytic leukemia (CLL). Copy number variation in the polyglutamine tract has been observed.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Under certain conditions, two single strands of nucleic acid with certain homology can be specifically hybridized to form double strands according to the principle of base complementarity. Generally, DNA molecules to be detected are digested with restriction enzymes, separated by agar-gel electrophoresis, denatured and transferred to nitrocellulocellulose film or nylon film or other solid phase support according to their position in the gel, fixed and then reacted with DNA probes labeled with isotopes or other markers. This is followed by autoradiography or an enzyme reaction to detect the amount of specific DNA molecules. If the object to be tested contains a sequence that is complementary to the probe, the two are combined by the principle of base complementarity, and the free probe is washed and detected by self-development or other suitable techniques, thus revealing the fragment to be tested and its relative size.

Applications

Gene Rearrangement Detection

Procedure

1. Sample Processing
2. DNA Extraction and Digestion
3. Gel Electrophoresis
4. Gel Pretreatment
5. Transfer membrane
6. Probe Labeling
7. Prehybridization (blocking)
8. Southern hybridization
9. Membrane washing
10. Autoradiographic Assay
11. Results Analysis

Materials

Sample: DNA, Bacterial Fluid/Tissue/Cell

Other recommended products

Analysis of ERG Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0015-LGZ)
Analysis of IL7R Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0077-LGZ)
Analysis of RANBP2 Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0096-LGZ)
Analysis of CTNNB1 Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0025-LGZ)
Routine Diagnosis of Large Granular Lymphocytic Leukaemia by Southern Blot Technology (CAT#: STEM-MHT-0125-LGZ)
Analysis of Pik3r1 Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0041-LGZ)
Analysis of DMD Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0010-LGZ)
Analysis of F8 Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0056-LGZ)