Analysis of PlGF (Human) by ELISA (CAT#: STEM-MB-1131-LGZ)

Introduction

Placental growth factor Placental growth factor, PlGF is a heparin-binding acidic protein, which can be synthesized and secreted by a variety of mesenchymal cells in the body, and secreted in the form of an inactive precursor. Its base sequence has high homology with VEGF gene, belongs to the vascular endothelial growth factor (VEGF) family, is abundantly expressed in the placenta, and exerts biological effects by binding to specific receptors. PlGF is not only a pregnancy-specific biomarker, it is also expressed in the heart, lung, thyroid, adipose tissue, and skeletal muscle, and its absence suppresses tumor growth and angiogenesis and arteriogenesis during heart, limb, and eye ischemia .

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine

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