Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of PP (Human, Non-human primate) by ELISA (CAT#: STEM-MB-1132-LGZ)

Introduction

Islet PP cells secrete pancreatic polypeptide (PP). PP was first discovered in chickens in 1968, and the entire family of structurally related peptides has been referred to as the pancreatic polypeptide family since then. However, in 1993, Larhammar et al. found that NPY was older in evolution and contributed more to evolution. Therefore, it is more appropriate to rename the pancreatic polypeptide family as NPY family. Like NPY, PP is also a polypeptide consisting of 36 amino acids with a molecular weight of 4179. Inhibit the secretion of insulin through paracrine and autocrine, and at the same time inhibit the secretion of pancreatic juice, especially inhibit the secretion of bicarbonate and trypsin, weaken the contraction of the gallbladder, strengthen the tension of the common bile duct, and inhibit the movement of the gastric antrum and small intestine. The release of PP is mainly inhibited by growth hormone, gastric inhibitory peptide and vasoactive intestinal peptide. PP not only has an effect on the exocrine of the pancreas, but also has an effect on the endocrine of the pancreas.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or supernatant
Advertisement