Analysis of PRDX6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0591-LGZ)

Introduction

Official Full Name: peroxiredoxin 6<br />Also known as: PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; LPCAT-5; HEL-S-128m<br />The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of ZFHX4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1866-LGZ)
Analysis of Maedi-Visna Virus (MVV) by Real-Time PCR Method (CAT#: STEM-MB-3185-LGZ)
Analysis of Francisella Novicida by Real-Time PCR Method (CAT#: STEM-MB-3585-LGZ)
Analysis of Bluetongue Virus (BTV) - 8 by Real-Time PCR Method (CAT#: STEM-MB-3113-LGZ)
Analysis of Madurella Spp. by Real-Time PCR Method (CAT#: STEM-MB-3768-LGZ)
Analysis of TMEM9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0725-LGZ)
Analysis of Equine Coronavirus (ECoV) by Real-Time PCR Method (CAT#: STEM-MB-3509-LGZ)
Analysis of KCNJ10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1082-LGZ)