Analysis of PRKD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1805-LGZ)

Introduction

Official Full Name: protein kinase D3
Also known as: EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
This gene belongs to the multigene protein kinase D family of serine/threonine kinases that bind diacylglycerol and phenolic esters. Members of this family are characterized by an N-terminal regulatory domain consisting of tandem repeats of cysteine-rich zinc finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. The catalytic activity of the enzyme facilitates its nuclear localization. The protein has been implicated in a variety of functions, including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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