Analysis of Prosthogonimus Macrorchis by Real-Time PCR Method (CAT#: STEM-MB-4054-LGZ)

Introduction

Prosthogonimus macrorchis, commonly referred to as the oviduct fluke, is a frequent parasite of many species of wild birds. The parasite is most abundant in regions of the United States and Canada near the Great Lakes. It is known for causing a reduction or termination in egg production in domestic fowl.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.