Analysis of PRUNE1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0581-LGZ)

Introduction

Official Full Name: prune exopolyphosphatase 1
Also known as: PRUNE; DRES17; HTCD37; NMIHBA; DRES-17; H-PRUNE
This gene encodes a member of the DHH protein superfamily of phosphatases. This protein has been found to function as a nucleotide phosphodiesterase and exopolyphosphatase. This protein is thought to stimulate cancer progression and metastasis by inducing cell movement. A pseudogene was found on chromosome 13. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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