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Analysis of PTEN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2056-LGZ)

Introduction

Official Full Name: phosphatase and tensin homolog<br />Also known as: BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta<br />This gene, identified as a tumor suppressor, is mutated at high frequency in a large number of cancers. The protein encoded by this gene is phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin-like domain and a catalytic domain similar to a dual-specificity protein tyrosine phosphatase. Unlike most tyrosine protein phosphatases, this protein preferentially dephosphorylates the phosphocarnosine substrate. It negatively regulates the level of intracellular phosphatidylinositol-3,4,5-triphosphate and exerts tumor suppressor effect by negatively regulating the AKT/PKB signaling pathway. Use of a non-canonical (CUG) upstream initiation site generates a longer isoform that initiates translation with leucine and is thought to be preferentially associated with the inner mitochondrial membrane. This longer isoform may help regulate mitochondrial energy metabolism. A pseudogene of this gene has been found on chromosome 9. Alternative splicing and the use of multiple translation initiation codons result in multiple transcript variants encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements