Analysis of ARNT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0790-LGZ)

Introduction

Official Full Name: aryl hydrocarbon receptor nuclear translocator<br />Also known as: ARNT1; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta<br />The protein encoded by this gene contains a basic helix-loop-helix domain and two characteristic PAS domains and a PAC domain. The encoded protein binds to the ligand-bound aryl hydrocarbon receptor and helps the complex move to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a cofactor in the transcriptional regulation of hypoxia-inducible factor 1. A chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 has been described in leukemia. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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