Analysis of CHML Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0867-LGZ)

Introduction

Official Full Name: CHM like Rab escort protein
Also known as: REP2
The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of EGR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2077-LGZ)
Analysis of POLR2D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1705-LGZ)
Analysis of Canine Adenovirus (CAV) by Real-Time PCR Method (CAT#: STEM-MB-2700-LGZ)
Analysis of LYVE1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2274-LGZ)
Analysis of ECEL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1765-LGZ)
Analysis of Japanese Encephalitis Virus by Real-Time PCR Method (CAT#: STEM-MB-2886-LGZ)
Analysis of FNDC10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1335-LGZ)
Analysis of ATOH8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1684-LGZ)