Analysis of RAB17 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1647-LGZ)

Introduction

Official Full Name: RAB17, member RAS oncogene family<br />The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of DENND4B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0923-LGZ)
Analysis of NOL8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1959-LGZ)
Analysis of MTHFR Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0078-LGZ)
Analysis of MRPL35 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1678-LGZ)
Rapid KRAS Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer by RT-qPCR (CAT#: STEM-MT-0015-LGZ)
Analysis of Carbapenem-resistant Klebsiella pneumoniae(R-Kp) by Real-Time PCR Method (CAT#: STEM-MB-2679-LGZ)
Analysis of Radix Saposhnikoviae by Real-Time PCR Method (CAT#: STEM-MB-3981-LGZ)
Analysis of RBP7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1400-LGZ)